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KMID : 1098420060140020063
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 2 p.63 ~ p.69
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Immuno-Regulatory Activities of an Isoflavone Glycoside, 4’, 6-Dimethoxylsoflavone-7-O-{beta}-D-Glucopyranoside and the Crude Extract Isolated from Amorpha fruticosa LINNE
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Kim Jung-Hwa
Kim Cheol-Hee
Kwon Min-Cheol
Kim Hyo-Sung
Lee Kang-Yoon
Lee Hyun-Jung
Kang Ha-Young
Lee Hak-Ju
Lee Hyeon-Yong
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Abstract
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The methanolic (MeOH) extract of A. fruticosa bark, which showed immune-regulatory activities, was separated to purify an active compared by means of a multi-stage column chromatography. This resulted in the isolation and characterization of an isoflavone glycoside named 4’’, 6-Dimethoxyisoflavone-7-O-{beta}-D-glucopyranoside. Immuno-regulatory activities of the crude extract of Amorpha fruticosa LINNE bark were compared with that of an isoflavone glycoside (4’’, 6-dimethoxyisoflavone-7-O-{beta}-D-glucopyranoside). The crude methanolic extract of A. fruticosa and purified single compound showed 16% of relatively low cytotoxicity at a maximum concentration of 1.0 g/L in cultivated normal human lung cell line (HEL299). Cell growth of human T cells was increased up to 15%, 0.5 g/L of the crude extract added group. This was higher than a single compound added one. On the other hand, specific production rates of IL-6 and TNF-{alpha} from T cell were higher in the purified compound treat group (0.82{times}10^{-4};pg/cell and 1.08{times}10^{-4};pg/cell, respectively), compared to 0.5 g/L of the crude extract added group (0.65{times}10^{-4};pg/cell and 0.84{times}10^{-4};pg/cell, respectively). In addition, the growth of NK-92MI cells incubated with the crude extract was higher up to 56% over the cells grown with a single compound (0.5 g/L). In overall, the crude extract showed relatively higher immuno-regulatory activities compared with a single compound, probably due to the synergic effect given by other substances existed in the crude extract. Even though the siolated compound stimulated higher secretion of cytokines from human T cells.
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KEYWORD
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Amorpha fruticosa L., 4’, 6-dimethoxyisoflavone-7-O-{beta}-D-glucopyranoside, Immuno-regulatory activity, cytokines, NK-92MI cell
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